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\begin{document}

\title{A novel Dhillonvirus phage bearing a unique open reading frame of
intergeneric origin}



\author[1]{Anastasios Vasileiadis}%
\author[2]{Petros Bozidis}%
\author[1]{Konstantinos Konstantinidis}%
\author[1]{Nikolaos Kesesidis}%
\author[3]{Louiza Potamiti}%
\author[4]{Anna Kolliopoulou}%
\author[4]{Apostolos Beloukas}%
\author[3]{Mihalis I. Panayiotidis}%
\author[2]{Konstantina Gartzonika}%
\author[1]{Ioannis Karakasiliotis}%
\affil[1]{Democritus University of Thrace}%
\affil[2]{University of Ioannina}%
\affil[3]{The Cyprus Institute of Neurology and Genetics Clinical Genetics Clinic}%
\affil[4]{University of West Attica}%


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  \date{May 28, 2024}


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\begin{abstract}
\emph{Escherichia Coli} ( \emph{E. coli}) is one of the most prevalent
bacteria in the gut and plays an important role in the formation of the
intestinal microbiome. In this study, Escherichia phage Ioannina, a
novel member of \emph{Caudoviricetes}, with high lytic activity against
\emph{E. coli} ATCC 25922, was isolated from hospital wastewater.
Escherichia phage Ioannina was highly resistant to a broad range of
temperatures and alkaline pH, but was sensitive to acidic pH. Whole
genome sequencing of the phage, revealed that Escherichia phage Ioannina
is a novel phage within the \emph{Dhillonvirus} genus. Its genome is a
45,270 bp double-stranded DNA molecule that encodes 64 putative open
reading frames (ORFs). The phylogenetic analysis of open reading frames,
which diversified Escherichia phage Ioannina from other dhillonviruses,
indicated that ORF31 (a putative tail fiber protein) presented higher
similarity to representatives of other phage families. ORF31 was
significantly more related to similar ORFs belonging to the
\emph{Kuravirus} and \emph{Tunavirus} ( \emph{Drexlerviridae}) genera,
indicating a possible recombination event.%
\end{abstract}%



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\verb`Manuscript Molecular Microbiology.docx`  available at \url{https://authorea.com/users/670077/articles/670677-a-novel-dhillonvirus-phage-bearing-a-unique-open-reading-frame-of-intergeneric-origin}

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