CXCL13 and IL-8 analysis
The concentration of CXCL13 in cerebrospinal fluid (CSF) was quantified utilizing a sandwich Enzyme-Linked Immuno Sorbent Assay (ELISA) technique, employing the Euroimmun CXCL13 ELISA CE kit (Euroimmun, Lübeck, Germany), with a catalog number EQ 6811-9602-L. The analysis was executed in compliance with the manufacturer’s guidelines, where values below 20 pg/ml were deemed within the normal range.
To measure IL-8 levels in CSF, an enzyme-labeled solid-phase chemiluminescent sequential immunometric assay was employed, specifically, the IMMULITE® 1000 IL-8 (Euroimmun, Lübeck, Germany) kit, catalog number LK8P1. The analysis was conducted following the manufacturer’s protocol, with CSF concentrations of up to 62 pg/ml considered as falling within the normal range.