Changes in skin microbiota composition |
Changes in skin microbiota
composition |
Do shifts in skin microbiota composition impact disease pathogenesis
or do they merely reflect underlying disease processes?
|
Mechanistic studies designed to assess the effect of changing skin
microbe abundances on disease pathogenesis
Intervention studies to understand role of microbes in disease
pathogenesis
|
How do skin microbiota interact with one another, and with the host to
influence disease pathogenesis?
|
Functional analysis of the contribution of microbes e.g. bacteria,
viruses, fungi and mites colonizing skin to skin disease
Use of deep sequencing methods integrated with culture methods and
measures of skin barrier function to determine the role of microbes in
disease pathogenesis
Standardization of protocols across skin phenotyping studies to allow
for comparison of data
Murine models investigating combinations of microbes, and deep
endophenotyping of host responses
|
Do skin microbiota alterations in early life impact AD and FA onset in
later childhood
If so, what are the mechanisms through which this might occur?
|
Larger longitudinal studies with long-term follow up, robust early
life skin barrier measures, and objective allergic outcomes
Mechanistic studies to validate the impact of changes in microbe
composition on disease onset
|
Skin microbe-based therapeutic approaches |
Skin microbe-based
therapeutic approaches |
Does therapeutic manipulation of skin microbiota prevent AD or FA or
reduce disease severity?
If so, what is the window of opportunity for such interventions?
|
Randomized controlled trials of early life skin microbiota
manipulation with long-term follow up for allergic disease onset
Detailed collection of skin barrier markers at serial time-points in
early life
|
Which patient groups would benefit from biotherapeutic interventions?
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Intervention studies to include different disease phenotypes e.g.
infected AD, positive colonization with target microbe, etc
|
Would disruption of pathogenic skin microbiota transmission between
close contacts improve outcomes?
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Randomized controlled trials of microbe eradication techniques
involving close contacts of index patients
|