2.8 Fabrication of the complete biomimicking trilayer tissue
engineering scaffolds
BMSC-laden GelMA/Gel bioinks were used to fabricate the cell-laden
hydrogel layer on S+F-PDA@E2 bilayered scaffolds via 3D bioprinting for
the construction of designed biomimicking trilayer tissue engineering
scaffolds. In the current study, GelMA/Gel inks were homogeneously mixed
with BMSCs at room temperature. The resulting cell-laden bioinks were
subsequently transferred to a cartridge in a 3D bioprinter. The printing
parameters were set as: the inner diameter of the printing nozzle was
210μm, the printing temperature was 20℃, and the printing speed was
6mm/s. After adjusting the height between the S+F-PDA@E2 scaffold and
printing nozzle, BMSC-laden GelMA/Gel hydrogels were 3D printed on
S+F-PDA@E2 scaffolds. Subsequently, a UV light (wavelength: 365 nm;
power: 365 mW) was used to crosslink the hydrogel layer for 2 min. The
trilayer tissue engineering scaffolds thus produced were transferred to
6-well cell culture plate and cultured with DMEM at 37 ℃. The
printability of BMSC-laden GelMA/Gel bioinks was evaluated by using the
following formula:
Printability = L2/16A (1)
where L is the perimeter and A is the area of the pore. If the
Printability value is close to 1, it signifies the square shape of the
pores, suggesting good printability of the ink or bioink[21].