Fig.1 Schematic illustration for the fabrication of trilayer tissue
engineering scaffolds for uterine tissue regeneration. (A) 3D printing
of the PTMC/TPU scaffold layer. (B) Electrospinning to fabricate the
E2-containing PLGA/GelMA fibrous layer on the PTMC/TPU scaffold layer.
(C) 3D bioprinting to construct the BMSC-laden hydrogel layer on the
PLGA/GelMA fibrous layer to complete the fabrication of designed
biomimicking trilayer tissue engineering scaffold.
Materials and methods
Materials
PTMC (molar ratio of LA:TMC = 8:2 and dl/g 0.9) and PLGA (molar ratio of
LA:GA =50:50 and average molecular weight of 90 kDa) were purchased from
Jinan Daigang Ltd., China. TPU 95A was supplied by BASF SE, Germany.
Estradiol (E2) was bought from Aladdin Co. Ltd., China. Gelatin (porcine
skin, type A), bovine serum albumin (BSA), methacrylate anhydride (MA,
94%), ammonium hydroxide solution (NH4OH) (28%),
absolute ethanol, 2-hydroxy-2-methylpropiophenone (97%),
1,1,1,3,3-hexafluoro-2-propanol (HFIP) and dopamine hydrocholoride
(98%) were bought from Sigma-Aldrich (St. Louis, MO, USA). Dialysis
tubing cellulose membrane (MWCO 10kDa) was supplied by Thermo Fisher
Scientific, USA. All reagents were used as received without further
purification.