2.9 Cell survival rate and shape morphing of trilayer tissue engineering scaffolds
Live/dead assay was used to examine the BMSC survival rate in the GelMA/Gel hydrogel layer. After printing and then culturing for 1, 3 and 7 days, respectively, BMSCs in the printed hydrogel layer were stained by live/dead assay according to the manufacturer’s protocol. Additionally, the BMSC distribution in the final trilayer tissue engineering scaffolds was visualized via CLSM. The shape morphing behavior of trilayer scaffolds was recorded using a digital camera when scaffolds were immersed in the culture medium at 37 ℃.