2.4.2 Data processing
Bacterial 16S rRNA gene sequences were analysed using the Quantitative Insights Into Microbial Ecology (QIIME) platform. First, the bidirectional sequences were spliced, the sequences with matching errors were checked, the chimeric sequences were removed, and high-quality sequences were obtained for the next analysis. According to UCLUST, all gene sequences were clustered according to 97% similarity, singleton OTUs were removed, and a representative OTU sequence was obtained. The species information was obtained by comparing the representative OTU sequences with the RDP reference database.