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\begin{document}

\title{Complete title: Value of a secretomic approach for distinguishing
patients with COVID-19 viral pneumonia among patients with respiratory
distress admitted to intensive care unit Shortened title: Secretomic to
distinguish COVID-19 patients}



\author[1]{Angélique Blangy-Letheule}%
\author[1]{Amandine Vergnaud}%
\author[1]{Thomas Dupas}%
\author[2]{Damien Habert}%
\author[1]{Jérôme Montnach}%
\author[3]{Walid Oulehri}%
\author[1]{Dorian Hassoun}%
\author[1]{Manon Denis}%
\author[1]{Jules Lecomte}%
\author[1]{Antoine Persello}%
\author[1]{Antoine Roquilly}%
\author[2]{José Courty}%
\author[4]{Michel Seve}%
\author[1]{Aurélia A. Leroux}%
\author[1]{Bertrand Rozec}%
\author[4]{Sandrine Bourgoin-Voillard}%
\author[1]{Michel De Waard}%
\author[1]{Benjamin Lauzier}%
\affil[1]{Nantes Universite}%
\affil[2]{Universite Paris-Est Creteil Val de Marne Faculte de medecine}%
\affil[3]{Les Hopitaux Universitaires de Strasbourg Hopital de Hautepierre}%
\affil[4]{Universite Grenoble Alpes Grenoble IAE}%


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  \date{May 26, 2024}


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\begin{abstract}
\emph{Introduction} \emph{:} In intensive care units, COVID-19 viral
pneumonia patients (VPP) present symptoms similar to those of other
patients with non-viral infection (NV-ICU). To better manage VPP, it is
therefore interesting to better understand the molecular pathophysiology
of viral pneumonia and to search for biomarkers that may clarify the
diagnosis. The secretome being a set of proteins secreted by cells in
response to stimuli represents an opportunity to discover new
biomarkers. \emph{Aims} \emph{:} Identify secretomic signatures of VPP
with those NV-ICU. \emph{Methods} \emph{:} Plasma samples and clinical
data from NV-ICU (n=104), VPP (n=30) or healthy donors (HD, n=20) were
collected at Nantes Hospital (France) upon admission. Samples were
enriched for the low-abundant proteins and analyzed using non-target
mass spectrometry. Specifically deregulated proteins (DEP) in VPP
\emph{versus} NV-ICU were selected. Combinations of 2 to 4 DEPs were
established. \emph{Results} \emph{:} The differences in secretome
profiles of the VPP and NV-ICU groups were highlighted. Forty-one DEPs
were specifically identified in VPP compared to NV-ICU. Five
combinations of 3 proteins with a receiver operating characteristic
curve presenting an area under the curve of 95.0\% were identified.
\emph{Conclusion}: This study identifies five combinations of candidate
biomarkers in VPP compared to NV-ICU that may help distinguish the
underlying causal molecular alterations.%
\end{abstract}%



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