Figure 4. Differential CD69 and CD103 expressing patterns defined CD4+ TRM cells into relatively functional distinct lineages.
(A) Flow plot indicated CD4+ TRM cells in fibrotic lungs were divided into four subsets by CD69 and CD103 expressions. CD69+CD103+ (Red); CD69+CD103 (Blue); CD69CD103+ (Orange); CD69CD103 (Green). (B-D) Flow histogram displayed transcriptional factor T-bet (B), GATA-3 (C), and ROR-γt (D) expressions among different subsets. Bar graph below showed the MFI among the indicated CD4+ TRMcell subsets (n = 6). (E) Flow histogram compared ratios of FOXP3+ among different subpopulations. Bar graph below revealed the percentages of FOXP3+ among the indicated subsets (n = 5). (F) Representative flow histogram compared the intensity of CD25, PD-1, ST2, ICOS, and CD39 among four subsets in CD4+ TRM cells. (G) Typical flow plots showed CD69 and CD103 expressing patterns on Teff cells (FOXP3) or Tregs (FOXP3+) in CD4+ TRM cells. (H) Typical flow plots demonstrated CD69 and CD103 expressing patterns on T-bet+, ROR-γt+, or GATA-3+ subsets in CD4+TRM cells. (I) The bar graph showed the ratios of CD103+ against CD103 subsets in CD69+CD4+ TRM cells (n = 5). Bar graphs are the combined results of at least three independent experiments. Individual mice are plotted on the graphs. Values are reported as the mean ± SD. P value is determined by one-way ANOVA followed by Tukey’s test.