Figure 4. Differential CD69 and CD103 expressing patterns
defined CD4+ TRM cells into relatively
functional distinct lineages.
(A) Flow plot indicated CD4+ TRM cells
in fibrotic lungs were divided into four subsets by CD69 and CD103
expressions. CD69+CD103+ (Red);
CD69+CD103– (Blue);
CD69–CD103+ (Orange);
CD69–CD103– (Green). (B-D) Flow
histogram displayed transcriptional factor T-bet (B), GATA-3 (C), and
ROR-γt (D) expressions among different subsets. Bar graph below showed
the MFI among the indicated CD4+ TRMcell subsets (n = 6). (E) Flow histogram compared ratios of
FOXP3+ among different subpopulations. Bar graph below
revealed the percentages of FOXP3+ among the indicated
subsets (n = 5). (F) Representative
flow histogram compared the intensity of CD25, PD-1, ST2, ICOS, and CD39
among four subsets in CD4+ TRM cells.
(G) Typical flow plots showed CD69 and CD103 expressing patterns on Teff
cells (FOXP3–) or Tregs (FOXP3+) in
CD4+ TRM cells. (H) Typical flow plots
demonstrated CD69 and CD103 expressing patterns on
T-bet+, ROR-γt+, or
GATA-3+ subsets in CD4+TRM cells. (I) The bar graph showed the ratios of
CD103+ against CD103– subsets in
CD69+CD4+ TRM cells
(n = 5). Bar graphs are the combined results of at least three
independent experiments. Individual mice are plotted on the graphs.
Values are reported as the mean ± SD. P value is determined by
one-way ANOVA followed by Tukey’s test.