2.1 Study setting and ethics statement
The P. aeruginosa AUST-03 (ST 242) strains investigated were
isolated from two CF patients attending clinics at a public tertiary
academic hospital in Johannesburg, South Africa. The two CF patients (P2
and P4) were part of a study that recruited 22 CF patients at two public
academic hospitals in Gauteng, South Africa over the period May 2019 to
February 2020. The study was granted ethical approval by the University
of the Witwatersrand Human Research Ethics Committee (Reference number:
M1811104) and by the University of Pretoria, Faculty of Health Sciences,
Research Ethics Committee (Reference number: 466/2018). Sputum specimens
were obtained from the CF patients following the provision of written
informed assent and consent from the CF patients and their parents,
respectively.