3.4 Polysaccharides, proteins, chlorophyll and carotenoids
Compared to the control, the soluble and cell surface proteins had no
significant change at Mt
treatments
(Fig. 4A). However, the content of intracellular protein increased
gradually with the elevation of Mt concentration, reaching 6.0 mg
10-12 cell at Mt200 and 7.6 mg
10-12 cell at Mt500. After Pal
addition, the soluble proteins rose from 1.6 mg 10-12cell to 2.0 and 3.0 mg
10-12 cells for Pal200 and
Pal500, respectively (Fig. 4A).
However, the intracellular
protein declined significantly from 5.5 to 3.7 mg
10-12 cells at Pal200 and increased
slightly to 4.0 mg 10-12 cells at
Pal500.
The contents of soluble and intracellular polysaccharides decreased with
the elevation of Mt, i.e., from 2.9 to 2.3 mg 10-12cells and from 7.2 to 5.8 mg 10-12 cells, respectively
(Fig. 4B). In contrast, these polysaccharides increased with the
elevation of Pal. Upon Pal500, the intracellular
polysaccharides increased remarkably from 7.2 to 13.4 mg
10-12 cells. The cell surface polysaccharides contents
hence showed an increasing trend under Pal addition.
The changes of chlorophyll a , chlorophyll b and
carotenoids in cells showed an upward trend under Mt and Pal addition
(Fig. 4C). Following the addition of Mt500, chlorophylla increased from 21.9 to 23.9 mg 10-12 cells,
whereas this value increased to 28.5 mg 10-12 cells at
Pal500. In
addition, upon the 500 mg/L Mt and Pal treatment, the content of
chlorophyll b rose from 12.2 to 15.3 mg 10-12cells and to 17.4 mg 10-12 cells, respectively. The
carotenoids content did not change significantly at
Mt500, but increased from 1.6 to 2.7 mg
10-12 cells at Pal500 (Fig. 4C).