3.7 Annotations of differentially expressed genes (DEGs)
Compared with control, differentially expressed genes (DEGs) were identified in the 200 mg/L Mt and Pal treatments. Since photosynthesis is an important physiological process in algae, we focused on those significantly different genes involved in photosynthesis (Figure. 8). IF2CP and CPLS1 genes were related to chloroplast synthesis based on the KEGG [35]. After the addition of Pal, these genes were down-regulated, which inhibited the chloroplast precursor material conversion to chloroplast. CAB4, CAB7 and CHLG genes regulated chlorophyll synthesis [36,37]. These genes were significantly upregulated compared with addition of 200 mg/L Mt. In addition, psbH and OHP1 genes related to PSII synthesis were significantly downregulated at Pal200 [38,39]. In addition, odhA and SAMC1 genes related to cellular respiration were upregulated at Pal200 [40,41]. However, the change was not evident in the gene expressions when Mt was added.