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\begin{document}

\title{Overexpression of AhTDC under waterlogging stress reduces kernel filling
rate due to crosstalk between melatonin and ethylene}



\author[1]{Yong Chen}%
\author[1]{Ruier Zeng}%
\author[1]{Tingting Chen}%
\author[1]{Xi Li}%
\author[1]{Jing Cao}%
\author[1]{Jie Li}%
\author[1]{Xueyu Xu}%
\author[1]{Lei Zhang}%
\affil[1]{South China Agricultural University College of Agriculture}%


\vspace{-1em}



  
  \date{May 24, 2024}


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\begin{abstract}
\textbf{Summary:} The hindrance of kernel development caused by
waterlogging stress (WS) is the direct reason for the peanut yield
reduction. Currently, the mechanism of kernel filling responding to WS
remains unknown. The waterlogging-sensitive variety Huayu 39 was
subjected to WS for 3 days after 7 days after gynophores touched the
ground (DAG), and the key stage of WS affecting kernel filling is 14,
21, and 28 DAG. WS decreased the average filling rate and kernel dry
weight. Therefore, transcriptome sequencing and widely-targeted
metabolomic analysis were conducted on kernel to elucidate the mechanism
for the decrease in average filling rate under WS, revealing that
overexpression of the gene encoding tryptophan decarboxylase (
\emph{AhTDC}), which caused the accumulation of melatonin, reduced
kernel weight. The sucrose transformation rate was limited by the
crosstalk between melatonin and ethylene, thereby reducing the kernel
filling rate and hindering kernel development. Our results are crucial
for formulating measures to alleviate the negative impact of WS on
peanut yield and quality, providing a basis for exploring high-yield and
high-quality cultivation, molecular-assisted breeding, and waterlogging
prevention.%
\end{abstract}%



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