2.8 Substrate scope investigation
To demonstrate the substrate range of CRL@OSMD, the esterification of
pine sterol with oleic, linoleic, linolenic acid, and mixed acid (oleic,
linoleic, and linolenic acids in equimolar ratios) was studied under
optimized reaction conditions.
2.9 Process
scale-up and reusability studies
To explore the scalability of the immobilized CRL-catalyzed pine sterol
ester synthesis process, the feed
volume was increased up to 10
times under optimized reaction conditions. The synthesis of pine sterol
easters was investigated for reusability under optimized conditions. At
the end of the reaction, the immobilized enzyme was filtered, separated
from the reaction system, and washed with 5 mL hexane 3 times. After
drying, the resulting immobilized CRL was used directly in the next
batch.