Endogenous expression of FLAG-tagged Vps8Dp and mNeon-tagged
genes in VPS8D knockdown strains
pVPS8D-FLAG-ZZ-Neo4, pDOP1-2mNeon-6myc-Neo4, pVMA4-2mNeon-6myc-Neo4 and
pSCR7-2mNeon-6myc-Neo4 (Cheng et al., 2023) were introduced by biolistic
transformation into strains in which the VPS8D hairpin expression
cassette was already integrated at the RPL29 locus, as described
above.