GFP reporter assays 
Single colonies of cells carrying the indicated reporter fusions were inoculated into sterile borosilicate glass tubes 16x125 mm (Fisher Scientific) containing 2 ml of TSB with appropriate antibiotic to maintain selection. Cells were grown to a stationary phase overnight. Pellets were collected and washed twice with PBS and resuspended in the same buffer. 100 μL of the cell suspension was transferred into a flat bottom black 96-well plate (Corning). Fluorescence was measured using a Synergy H1 plate reader (BioTek/Agilent) tuning the monochromator to 485 nm and 535 nm (excitation/emission, respectively). Relative fluorescence units (RFUs) were calculated by subtracting the fluorescence of plain PBS and dividing by OD600 to correct for cell density.