Analysis of fatty acid compositionĀ
S. aureus strains were grown to exponential phase
(OD600 ~ 0.5), at which time 10-50 ml of
each culture (~100 mg of wet cells) were pelleted,
washed twice with phosphate-buffered saline (PBS), and stored at -80°C.
Fatty acids were saponified and methylated, and then analyzed by gas
chromatographic analysis of fatty acid methyl esters (GC-FAME) as a fee
for service at the Center for Microbial Identification and Taxonomy
(Norman, OK).