Kinetic analysis
Reactions were performed using the acyl-CoA synthetase activity assay
described above. Pseudo-first order kinetic parameters were calculated
using Prism version 9.0 (GraphPad Software), fitting the data to
Equation 1,
Vo = V x [S] /
(Km + [S]) (Equation 1)
where Vo is initial velocity, [S] is
substrate concentration, V is maximum velocity, and KMis the Michaelis-Menten constant (Segel, 1975).