Statistical analyses 
Prism version 9.0 (GraphPad Software) was used to perform all statistical analyses. Statistical significance was calculated using measurements from at least three biological replicates and one-way or two-way analysis of variance (ANOVA); for time course experiments, two-way ANOVA with repeated measures was used to assess differences and, if significant, analyses were followed by Tukey’s honestly significant difference (Tukey’s HSD) tests for pairwise comparisons. Statistical significance was assumed at a P value of <0.05.