Statistical analyses
Prism version 9.0 (GraphPad Software) was used to perform all
statistical analyses. Statistical significance was calculated using
measurements from at least three biological replicates and one-way or
two-way analysis of variance (ANOVA); for time course experiments,
two-way ANOVA with repeated measures was used to assess differences and,
if significant, analyses were followed by Tukey’s honestly significant
difference (Tukey’s HSD) tests for pairwise comparisons. Statistical
significance was assumed at a P value of <0.05.