Histological sections of Peyer’s patches from male Wistar rats of the groups Control - CT (A), Bisphenol A - BPA (B), Bisphenol A + Selenium - BPA + Se (C) and Selenium - SE (D) stained by Masson’s trichrome method. The presence of lymphocytes (arrowhead), leukocyte groups (circle) and macrophages (*) was observed in all groups studied. Scale bars: 1000x magnification.
At higher magnification (1000x), leukocytes of varying sizes and staining intensities were observed. After measurement, it was observed that there was a significant reduction (p < 0.05) in the number of defense cells in the PP’s in the BPA group (30.08±1.87) when compared to the other groups (TC: 38.72±3.30; BPA+Se: 37.56±2.61; If: 38.36±1.98) (Figure 6). However, a greater presence of macrophages was observed in the BPA and BPA+Se groups. No significant differences in the number of immune cells were observed in the other groups.
Figure 6. Number of leukocytes in Peyer’s patches.