Statistics
All statistical analyses were performed using Graphpad Prism version 9 software (GraphPad Software, LLC, San Diego, CA, USA). An unpaired two-tailed Student’s t-test was used to compare differences between two groups when data had a Gaussian distribution with similar variances. To compare multiple groups, one-way analysis of variance (ANOVA) was performed followed by Tukey’s post hoc test. Values of p< 0.05 were considered significant.