Differential gene screening and identification
DE genes after treatment with different doses of ag85a/b DNA
vaccine immunized with IM or EP were screened by a hierarchical
clustering heat map, scatter plot, and volcano plot analysis. The DE
genes were compared between the TB model group and the normal control
group and between each ag85a/b DNA treatment group and the TB
model group. A Hierarchical cluster plot shows differences in gene
expression among different groups of samples. The scatter plot shows the
DE gene correlation between the two groups of samples, where the x-axis
values and the y-axis values are mean-normalized (log2 scaling)
processed results. The volcano plot shows the distribution of DE genes
between the two groups of samples, and the x-axis represents the log2
value (Fold Change), and the y-axis represents the -log10
value(P-value).