PBMC isolation and total RNA extraction
In this study, the whole blood from 3 mice in each group mentioned above
was collected in an ethylenediaminetetraacetic acid dipotassium (EDTA)
anticoagulant tube three weeks after the last immunization, and then
isolated peripheral blood mononuclear cells (PBMCs) with the Mouse PBMC
Isolation Kit (Haoyang Biological Products Technology, Tianjin, China).
Total RNA was extracted from PBMCs using TRIzol Reagent (Life
Biotechnology, Shanghai, China) according to the manufacturer’s
instructions.