|
|
TB model group vs Normal group |
DNA IM group vs TB model group |
DNA IM group vs TB model group |
DNA IM group vs TB model group |
DNA IM
group vs TB model group |
DNA EP group vs TB model group |
DNA EP group
vs TB model group |
DNA EP group vs TB model group |
DNA EP group vs TB
model group |
|
|
|
|
10μg |
50μg |
100μg |
200μg |
10μg |
50μg |
100μg |
200μg |
|
NM_011359 |
Sftpc |
167↓ |
NO |
NO |
559↑ |
395↑ |
255↑ |
289↑ |
565↑ |
280↑ |
Surfactant protein C, a hydrophobic protein secreted by alveolar
epithelial cells, maintains lung tissue stability by reducing the
surface tension of the fluid covering the lungs(89). |
NM_001282071 |
Sftpb |
68↓ |
NO |
NO |
348↑ |
253↑ |
100↑ |
129↑ |
207↑ |
99↑ |
Surfactant protein B, an amphoteric surfactant protein secreted
by alveolar epithelial cells, increases the diffusion rate and stability
of the surfactant layer in vitro(90). |
NM_009160 |
Sftpd |
48↓ |
NO |
NO |
141↑ |
60↑ |
59↑ |
78↑ |
146↑ |
98↑ |
Surfactant protein D, a member of the collectin family, is soluble
innate immune molecule which maintain lung homeostasis through their
dual roles as anti-infectious and immunomodulatory agents(91,
92). |
NM_009349 |
Inmt |
43↓ |
NO |
NO |
164↑ |
154↑ |
39↑ |
36↑ |
223↑ |
116↑ |
Indolethylamine N-methyltransferase, is a methyltransferase that
regulates the N-methylation of tryptamine family proteins, participates
in the development and activity of the nervous
system(93). |
NM_023134 |
Sftpa1 |
32↓ |
NO |
NO |
127↑ |
102↑ |
64↑ |
50↑ |
127↑ |
75↑ |
Surfactant protein A1, a member of type C lectin subfamily, plays
an important role in surfactant homeostasis and defense against
respiratory pathogens, and mediates adhesion and phagocytosis of MTB by
alveolar macrophages (66). |
NM_010329 |
Pdpn |
32↓ |
NO |
NO |
57↑ |
27↑ |
38↑ |
66↑ |
55↑ |
41↑ |
Podoplanin, a small transmembrane mucin-like glycoprotein that plays an
important role in immune response. In different inflammation-related
diseases, the expression of Pdpn in immune cells participates in the
regulation of inflammation(94). |
NM_026323 |
Wfdc2 |
32↓ |
NO |
NO |
117↑ |
66↑ |
53↑ |
73↑ |
113↑ |
70↑ |
WAP four-disulfide core domain 2, a small secreted protein that
functions as a protease inhibitor, plays critical roles in multiple
aspects of lung function, such as promoting mucociliary clearance,
conferring anti-inflammatory activity, and reducing surface
tension(95). |
NM_001029937 |
Sec14l3 |
28↓ |
NO |
NO |
116↑ |
185↑ |
83↑ |
86↑ |
237↑ |
139↑ |
SEC14-like 3, a 45kDa
secretory protein specifically expressed in airway epithelial cells, has
a close relationship with airway inflammation, and decreases
significantly with the aggravation of airway inflammation, which may be
a new marker of airway inflammation(96, 97). |
NM_001198766 |
Postn |
27↓ |
NO |
NO |
87↑ |
52↑ |
46↑ |
79↑ |
101↑ |
71↑ |
Periostin, a 90-kDa secreted extracellular matrix protein, binds
to many extracellular matrix proteins through its different domains, and
can bind to diverse integrins to activate the TGF-β, PI3K/Akt, Wnt,
RhoA/ROCK, NF-κB, MAPK, and JAK pathways(98). |
NM_013805 |
Cldn5 |
23↓ |
NO |
NO |
52↑ |
28↑ |
19↑ |
36↑ |
66↑ |
37↑ |
Claudin 5, a member of the Claudin family, is an integral membrane
protein and a component of tight junctions, which act as physical
barriers preventing free passage of solutes and water through the
paracellular space between epithelial or endothelial cell
sheets. |
NM_007817 |
Cyp2f2 |
22↓ |
NO |
NO |
166↑ |
209↑ |
54↑ |
48↑ |
314↑ |
138↑ |
Cytochrome P450, family 2, subfamily f, polypeptide 2, is a
monooxygenase that catalyzes many reactions in drug metabolism and
metabolizes a variety of pulmonary toxicants(99). |
NM_009701 |
Aqp5 |
22↓ |
NO |
NO |
31↑ |
18↑ |
22↑ |
25↑ |
35↑ |
31↑ |
Aquaporin 5, a small water channel membrane proteins associated with
major intrinsic proteins (p38, MIP etc.), mediates cell migration and
proliferation, and participates in inflammatory
reaction(100). |
NM_007929 |
Emp2 |
21↓ |
NO |
NO |
64↑ |
47↑ |
41↑ |
45↑ |
77↑ |
53↑ |
Epithelial membrane protein 2, a member of the tetraspan superfamily of
membrane protein, has a variety of functions, including endocytosis,
cell signaling, proliferation, migration, and
adhesion(101). |
NM_020509 |
Retnla |
21↓ |
NO |
NO |
24↑ |
NO |
27↑ |
95↑ |
12↑ |
18↑ |
Resistin-like alpha, a member of
the resistin family, is a secreted cysteine-rich protein with insulin
resistance and also an anti-inflammatory marker of macrophages(78,
79). |
NM_011315 |
Saa3 |
20↓ |
NO |
NO |
NO |
NO |
NO |
56↑ |
NO |
NO |
Serum
amyloid A3, a pseudogene in humans, is a major component of acute-phase
inflammation, serves as an endogenous peptide ligand for TLR4, and binds
MD-2 to activate p38 and NF-κB pathways in a MyD88-dependent
manner(102). |
NM_010426 |
Foxf1 |
17↓ |
NO |
NO |
86↑ |
68↑ |
26↑ |
39↑ |
124↑ |
59↑ |
Forkhead box F1, a mesenchymal transcriptional factor essential for
lung development, promotes normal lung homeostasis and
repair(103). |
NM_008485 |
Lamc2 |
17↓ |
NO |
NO |
22↑ |
14↑ |
23↑ |
27↑ |
24↑ |
19↑ |
Laminin subunit gamma 2, laminin is an extracellular matrix glycoprotein
and the important adhesive component of the epithelial basement
membrane. They are involved in various biological processes such as cell
adhesion, differentiation, migration, signaling, neurite outgrowth and
metastasis(104). |
NM_008597 |
Mgp |
17↓ |
NO |
NO |
32↑ |
21↑ |
26↑ |
48↑ |
40↑ |
27↑ |
Matrix gla protein, a vitamin K-dependent inhibitor of calcification,
may play an anti-inflammatory role in monocytes and
macrophages(105). |
NM_008344 |
Igfbp6 |
16↓ |
NO |
NO |
58↑ |
38↑ |
19↑ |
15↑ |
66↑ |
39↑ |
Insulin-like growth factor binding protein 6, prolongs the half-life
of insulin-like growth factor (IGF), inhibits or stimulates the
growth-promoting effect of IGF in cell culture, activates the MAPK
signaling pathway, and induces cell migration (106) |
NM_010217 |
Ctgf |
15↓ |
NO |
NO |
31↑ |
18↑ |
25↑ |
50↑ |
53↑ |
44↑ |
Connective tissue growth factor, a 38-kDa protein with a tetramodular
structure, is involved in the basic lung development process and
promotes lung fibroblast proliferation, migration, and
differentiation(107). |