Drug treatment and assessment of TNBS-induced colitis
For the induction of TNBS-induced experimental colitis, C57BL/6 mice
were randomly divided into three groups, including a normal group, a
vehicle control group, and a drug treatment group that received 2.5%
2,4,6-trinitro-Benzenesulfonic acid (TNBS) by clysma on the first day of
the experiment. The drug treatment group received 40mg/kg PHI orally.
The body-weight loss ratio and survival rate were monitored. At the end
of the experiment, mice were anesthetized using intraperitoneal
injection of 4 % chloral hydrate. The peripheral blood, colon, and
mesenteric lymph nodes were collected for subsequent analysis.