##Reduced overload of iron by ferroptosis inhibition
Iron overload plays an important role in oxidative stress, and the accumulation and lipid peroxidation of Fe2+ are the key factors of ferroptosis. To explore SRS 16-86 on overload of iron on DN, an iron ion detection kit was used to measure the iron content. In this study, the overload of iron in the DM group was significantly increased compared with that in the control group, while SRS 16-86 treatment could reduce the content of iron compared with the DN group, indicating a reduced overload of iron (Figure 3A).