2. Material and methods
Particular attention was paid to the variability of the vegetative organs in different periods of ontogeny, which is of diagnostic importance for determining the sectional and species affiliation (e.g., Levichev 1999a, 1999b, 2011, Peterson et al. 2008; Peruzzi et al. 2008). Also, these features were analyzed on all herbarium sheets found in TARI and LE. To obtain mature seeds, live samples were placed in a container and observed in a greenhouse. Identification was carried out according to existing literature sources (Wendelbo and Rechinger 1990; Levichev 2006; Ali and Levichev 2007; Zarrei et al. 2007, 2011). Additionally, the anatomical features of the bulb, bulbil and leaves were studied from the material fixed in ethyl alcohol (70%). Manually, with a razor blade, longitudinal sections of the bulb, bulbil and cross sections of various parts of the basal leaf and peduncle were made. Sections were bleached in sodium hypochlorite for several minutes, then stained twice with cellulose carmine (phloem) and dilute methyl green (xylem). The anatomical features of the leaf were examined under a light microscope Nikon BX53 (Japan) Nikon BX53 (in TARI) and Stemi 2000 SDA (in LE) with a digital camera mounted on it. The images were then converted into graphics using Adobe Illustrator CC. 17.0.