3.6 The effect of Tube on reprogramming sugar metabolism in RAW
264.7 cells
Tube inhibited LPS-induced glycolysis in RAW264.7 macrophages
(Figure 8 ) and reduced basal and maximal respiration
(Figure 9 ). This finding suggests that Tube inhibits glycolysis
in RAW264.7 macrophages at the level of mitochondrial oxidative
phosphorylation. The transcript levels of critical glycolytic
rate-limiting enzyme genes were measured using RT-PCR. Tube increased
the transcript levels of GLTU1, PFKFB3, and hexokinase 2 (HK2), while
inhibiting the transcript levels of LDHA (Figure 10 ). Pyruvate
content in RAW264.7 macrophages increased with the application Tube
(Figure 11 ).