2.1 Animal
Male C57BL/6 mice at 7–10 weeks, weight 20–25 g, were purchased from Xi’an Jiaotong University Experimental Animal Center (originally from the Jackson Laboratory), animal number JAX strain code 000664. The mice were maintained at 24–26 °C, relative humidity of 45%–60%, and a diurnal cycle time of 12:12 hours. They were permitted access to food and water ad libitum. The mice were randomly divided into three groups of ten each, group 1: phosphate-buffered saline (PBS) treatment (Control group); group 2: 10 mg/kg of LPS treatment (LPS group); group 3: 10 mg/kg LPS + 1 mg/kg Tube treatment (Tube group). The Institutional Animal Care and Use Committee approved the study, and the animal welfare regulations established by the Laboratory Animal Center of Xi’an Jiaotong University were strictly observed.