2.3 Construction of a mouse model of acute inflammation
All mice fasted for 12 hours before the start of the experiment but drank water ad libitum. The Tube group was treated intraperitoneally 3 hours before modeling, while the control and model groups were injected intraperitoneally with an equal volume of saline only. After 3 hours, the model and Tube groups were given LPS (10 mg/kg) intraperitoneally, and the results of hematoxylin and eosin H&E staining judged the success of the modeling. After the injection of each group, mice were placed in cages and allowed to eat and drink ad libitum. The general activity was recorded. Six hours later, the animals were sacrificed, the chest was quickly opened, the organs were removed, and filter paper was used to blot dry surfaces for photography. Then, 0.5 g of tissue samples were homogenized in 1 ml PBS using an ultrasonic disintegrator. The supernatants were centrifuged at 5000 g for 5 min at 4 °C and used for subsequent analysis.