3.1 Effect of Tube on the gross morphology and histology in
mouse organs
As shown in Figure 1 , in the LPS-induced acute inflammation
model (see reference[7] for the concentration of LPS), the spleen of
the model group was significantly congested with a dark color and
blunted rim compared, indicating hyperfunction after LPS treatment. In
contrast, the Tube group had less congestion and a relatively lighter
color than the model group; however, the overall appearance was similar
to that of the control group. This finding suggests that Tube prevents
LPS-induced splenic congestion.
The Tube group had significantly fewer erythrocytes in the splenic red
marrow, fewer macrophages in the germinal centers, less inflammatory
infiltration of the alveolar wall in the lungs, reduced alveolar wall
thickness, and reduced structural damage, suggesting that Tube
significantly attenuated LPS-induced histomorphological changes in the
spleen and lungs, as shown in Figure 2 . In addition, the LPS
sets by looking at Figures 1 and 2 also show that our model construction
was successful.