METHOD
A mouse model of LPS-induced acute inflammation was constructed, and the protective effect of tubeimoside III against LPS-induced injury was investigated using histochemistry and real-time quantitative PCR. Western blotting, Seahorse extracellular flux analyser assays, and pyruvate content assays were used in LPS-induced RAW264.7 cells to explore how tubeimoside III exerts its anti-inflammatory effects. The potential mechanism was also validated using inhibitors.