Figure 12. Compared with the LPS model group at each period,
the mRNA levels of Nfkbiz (the transcriptional counterpart sequence of
IκBζ) and its downstream molecule IL-1α were significantly lower in the
Tube group. The mRNA levels of Nrf2, NQO1, HO-1, and GCLM were
significantly higher, while the JAK1, STAT6, and TET2 mRNA levels were
significantly lower. *P < 0.05,**P < 0.01, ***P
< 0.001, ****P < 0.0001. Values
are expressed as mean ± SEM, and each experiment was repeated three
times.
3.8 Effect of Tube on the
expression of I-kappa B Zeta (IκBζ) protein in RAW 264.7 cells
We measured the changes of ATF3 and IκBζ protein levels in LPS-treated
RAW264.7 macrophages by western blot (Figure 13 ). Compared with
the LPS model group, ATF3 protein levels were significantly higher, and
IκBζ protein levels were significantly lower in the Tube group,
regardless of whether LPS was added for one or more hours. These
findings suggest that Tube increased the expression of ATF3 and
inhibited the expression of IκBζ protein, consistent with the RT-PCR
experiment described above.