2.7 Cytokine assay
ELISA kits (Mouse TNFα,
Cat# BMS607-3TEN; Mouse IL-6, Cat#
BMS603-2; Mouse IL-1β,Cat#88-7013A-88) were purchased from Thermo
Fisher (USA).
2.8 Seahorse
extracellular flux analyser assays for ECAR and OCR
The glycolysis rate, spare respiratory capacity, and mitochondrial
respiration in RAW264.7 cells were measured using a Seahorse Glycolysis
Stress Test Kit (103020-100, Agilent) and a Seahorse Cell Mito Stress
Test Kit (103015-100, Agilent) and according to the manufacturer’s
instructions. Oxygen consumption rate and extracellular acidification
rate were measured on an XFe 96 Seahorse Bioanalyzer (Agilent). Data
were analyzed using Wave software (Agilent Technologies).