2.2 Cells
The cell line was derived from the mouse mononuclear macrophage leukemia cell line RAW264.7 (a gift from the Second Affiliated Hospital of Xi’an Jiaotong University). DMEM (Nerzerum, New Zealand) was supplemented with 10% fetal bovine serum Gibco, USA), 100 U/ml penicillin (Amresco, USA), and 100 μg/ml streptomycin (Amresco, USA) and incubated at 37 °C in a 5% CO2 environment. A Cell Counting Kit-8 (CCK-8, Cat# E606335, Biotech, China) was used to determine the optimal density for cell inoculation.