Verification differentially expressed miRNAs
Sequencing results show the expression of mmu-miR-30e-3p,
mmu-miR-203-3p, mmu-miR-125b-5p and mmu-miR-30c-2-3p were downregulation
and the expression of IL-1rn, Ctss, Was and Prr5l were obviously
upregulation(Fig 5A). In order to evaluate
the reliability of the sequencing results, the screened miRNAs and mRNAs
were verified by RT-qPCR. The results showed that compared with the
control group, the expression of mmu-miR-30e-3p, mmu-miR-203-3p,
mmu-miR-125b-5p and mmu-miR-30c-2-3p in the liver of infected mice were
significantly reduced, and the expression levels of IL-1rn, Ctss, Was
and Prr5l were significantly increased(Fig
5B-C). The RT-qPCR results is consistent with the sequencing results.