Quantitative real-time PCR analysis
Following the manufacturer’s instructions, total RNA was isolated from
mouse liver using Trizol reagent (Invitrogen, USA). And then
reverse-transcribed into cDNA using the qPCR RT kit(GeneCopoeia,
USA),The cDNA was analyzed using real-time qPCR with a StepOnePlusTM
Real-Time PCR System(Thermofisher, USA). Each reaction was performed in
triplicate in a 96-well plate. The expression of each gene was
normalized by the expression of GAPDH. miRNA and mRNA primers are listed
in Table 1.
Table 1. The sequences of miRNA and mRNA
primers