Target prediction of the differential miRNAs
Differential miRNAs target gene prediction was performed using both software A and B and target genes predicted by both software were screened according to scoring criteria. target genes with context score percentage less than 50 are removed in targetscan algorithm. Target genes with a maximum free energy (Max Energy) greater than -10 are removed in miRanda algorithm (i.e. the threshold is TargetScan score ≥ 50, miranda_Energy < - 10). Finally the intersection of these two software was taken as the final target gene of the differential miRNAs.