Verification differentially expressed miRNAs
Sequencing results show the expression of mmu-miR-30e-3p, mmu-miR-203-3p, mmu-miR-125b-5p and mmu-miR-30c-2-3p were downregulation and the expression of IL-1rn, Ctss, Was and Prr5l were obviously upregulation(Fig 5A). In order to evaluate the reliability of the sequencing results, the screened miRNAs and mRNAs were verified by RT-qPCR. The results showed that compared with the control group, the expression of mmu-miR-30e-3p, mmu-miR-203-3p, mmu-miR-125b-5p and mmu-miR-30c-2-3p in the liver of infected mice were significantly reduced, and the expression levels of IL-1rn, Ctss, Was and Prr5l were significantly increased(Fig 5B-C). The RT-qPCR results is consistent with the sequencing results.