Preparation of recombinant pB602L
To prepare purified recombinant pB602L for mouse immunization, the B602L
gene of ASFV (Pig/HLJ/2018) was cloned into pGEX-6p-1 vector and
expressed as GST-tagged pB602L protein in E. coli BL21(DE3). The
expression and purity of the recombinant pB602L protein were assessed by
SDS-PAGE firstly. Specific bands corresponding to GST-pB602L and pB602L
were observed (Fig. 2A). The expressed pB602L was further identified by
Western blot assays using an anti-GST monoclonal antibody (Fig. 2B) and
an anti-ASFV pig serum (Fig. 2C). Consensus results were obtained by
SDS-PAGE and Western blot. These analyses also revealed 95% purity of
the final purified recombinant protein (Fig. 2A, C), indicating the
purified pB602L protein was suitable for mouse immunization.