2.2. RNA extraction and cDNA synthesis
The fecal and ascetic fluid samples were fully resuspended in
phosphate-buffered saline (1:5 w/v) and centrifuged at 10,000 × g for 10
min. According to the manufacturer’s instructions, viral RNA was
extracted from 300 μL of the fecal suspension using RNAios Plus (TaKaRa
Bio Inc). The cDNA was synthesized using the PrimeScript™ RT Reagent Kit
according to the manufacturer’s instructions (TaKaRa Bio Inc) and then
stored at –20 °C until required.