Legends:
Figure 1: Distribution of different immune subsets among study participants (A-B) Variation across seronegative (SN), asymptomatic mild (AM), symptomatic mild (SM), moderate (MD) and severe (SV) groups in absolute count and frequency of CD4+ T cells subsets (N= Naïve, CM= central memory, EM= Effector Memory and TD= Terminally differentiated), respectively. (C-D) Variation across different study groups in absolute count and frequency of CD8+ T cells subsets, respectively. (E-F) Variation across different groups in Tregs count (E) and frequency(F). (G) Variation in frequency of monocyte subsets across different study groups. Statistical significance was calculated by Mann-Whitney U-test; *, p < 0.05; **, p < 0.01, ***, p<0.001 ; and ****, p<0.001.
Figure 2: Evaluation of IL-6 and sMAdCAM along with correlation of cellular immune subsets in study participants Variation in levels of (A) IL-6 and (B) sMAdCAM in seronegative(SN), asymptomatic mild (AM), symptomatic mild (SM), moderate (MD) and severe (SV) groups. Relationship between (C) IL-6 and sMAdCAM and their association with different cellular subsets (D) Lymphocyte count (E) Total T cell count (F) CD4+ T cell count (G) CD8+ T cell count (H) B cell count (I) NK cell count. Statistical significance was calculated by Mann-Whitney U-test; *, p < 0.05; **, p < 0.01, ***, p<0.001 ; and ****, p<0.001. Correlation analysis was performed using non parametric Spearman Rank Correlation test.
Figure 3: Distribution of IL-15 and its correlation with immune cells among study participants. Variation in levels of IL-15 (A) and detection rate (B) in seronegative (SN), asymptomatic mild (AM), symptomatic mild (SM), moderate (MD) and severe (SV) groups. Association of IL-15 and with different cellular subsets (C) Lymphocyte count (D) Total T cell count (E) CD4+ T cell count (F) CD8+ T cell count (G) B cell count (H) NK cell count (I) Soluble MAdCAM. (J-L) Kaplan Meier plots depicting hospitalization duration post recruitment associated with plasma levels of (J) IL-15 (K) sMAdCAM and (L) IL-6. Individuals were categorized into a low and a high group on the basis of median levels. Statistical significance was calculated by Mann-Whitney U-test; *, p < 0.05; **, p < 0.01, ***, p<0.001; and ****, p<0.001. Correlation analysis was performed using non parametric Spearman Rank Correlation test. For Kaplan Meier comparisons, Log-rank (Mantel-Cox) test was used.