Real-time PCR detection of SARS-CoV-2
Over 240 swab samples were obtained from the upper respiratory tracts of
participants at different time points throughout hospitalization. The
presence of SARS-CoV-2 was detected by qRT-PCR assay as previously
reported. Briefly, the nucleocapsid protein and open reading frame 1ab
were amplified and examined with two pairs of primers. Each sample was
detected in triplicate with positive and negative controls. The
diagnostic criteria were based on the recommendations by the National
Centers for Disease Control and Prevention of China.