Materials
TAM (#T5648), 4HT (#H7904), E2 (#E8875) and LPS (fromEscherichia Coli O111:B4; #L4130) were purchased from
Sigma-Aldrich (St. Louis, Missouri, USA). TAM, 4HT and E2 were dissolved
in EtOH (#20821.321, VWR,
Radnor,
Pennsylvania, USA) to a stock concentration of 10-2M. LPS was dissolved in sterile H2O to a stock
concentration of 1 mg/mL. G1 (#3577) was purchased from Tocris
(Bristol, UK) and dissolved in DMSO (#D8779, Sigma-Aldrich) to a stock
concentration of 10-2 M. Ly294,002 (#278-038) was
obtained from Alexis Biochemicals (Axxora LLC, San Diego, USA).
Phosphatase (#A32957) and Protease (#A32953) Inhibitor Mini Tablets,
Blue Coomassie Plus Bradford Assay Reagent (#23238), Bovine Serum
Albumin Standard 2 mg/mL (#23209) were purchased from Pierce (Waltham,
Massachusetts, USA). MTT (Thiazolyl Blue Tetrazolium Bromide, #M5655)
was purchased from Sigma-Aldrich. Primary antibodies used in Western
blotting are listed in Supplementary Table 2. Secondary HRP-conjugated
antibodies used as 1:2000 dilutions were purchased from Vector
Laboratories (Burlingame, California, USA), specifically goat
anti-rabbit IgG (#PI-1000) for primary antibodies against NRF2, HMOX-1,
pAKT and AKT, and HRP horse anti-mouse IgG (#PI-2000,) for Caspase-1
and β-actin.