Polymersomes uptake
Peritoneal macrophages from heterozygous RosaTdTomato female mice were
seeded at the density of 105 cells/well on 8 microwell
glass-bottom petri dishes (ibidi). After 24 h, cells were treated for 3
h as specified and then incubated with Cy5-labelled nanoparticles for 1
and 3 hours. After medium discharge, cells were washed 3 times in PBS to
remove any excess of nanoparticles and fixed in formaldehyde (4%) for
10 minutes, then washed and analysed by confocal microscopy (ZEISS Axio
Observer). Fluorescence intensity of polymersomes inside macrophages was
scored by ImageJ. The number of polymersomes-positive cells was counted
in 4 fields/well, with an average of 200 total cells counted in each
well.