H19 and miR-29b regulates MCAO-induced C1QTNF6, IL-1β, and TNF-α expression
To determine whether miR-29b is involved in the pathophysiology of cerebral ischemic stroke, MCAO rats were intravenously injected with miR-29b antagomir to decrease miR-29b expression in leukocytes. First, miR-29b antagomir treatment significantly increased C1QTNF6 mRNA levels in leukocytes of MCAO rats (Figure 3A, P < 0.05). Moreover, miR-29b antagomir treatment increased C1QTNF6 protein levels in the brain (Figure 3B,P < 0.05). Additionally, miR-29b antagomir treatment increased IL-1β and TNF-α levels in MCAO rats (Figure 3A, B, P< 0.05).
Additionally, we analysed IL-1β and TNF-α expression in MCAO rats treated with H19 siRNA. Treated with H19 siRNA significantly increased brain and leukocyte levels of IL-1β and TNF-α (Figure 3A, B, P< 0.05). Additionally, C1QTNF6 mRNA and protein expression in leukocytes and the brain, respectively, were significantly downregulated in MCAO rats treated with H19 siRNA (Figure 3A, B, P < 0.05). Taken together, our findings suggest that H19 and miR-29b regulate C1QTNF6 levels; moreover, they affect IL-1β and TNF-α expression in leukocytes and the brains of MCAO rats.