2,3,5‐Triphenyl‐2H‐tetrazolium chloride (TTC) staining
Rats were sacrificed 24 h after reperfusion. Brains were quickly removed
and cut into 2-mm thick coronal slices for TTC staining. Infarct and
oedema volume were detected using TTC staining. The infarct volume was
calculated as follows: (contralateral hemisphere volume - undamaged
ipsilateral hemisphere)/contralateral hemisphere volume × 100%. Volume
calculation with oedema correction as follows: (ipsilateral hemisphere
volume-contralateral hemisphere volume)/contralateral hemisphere volume
× 100%.