ELISA
We collected plasma from patients with stroke and healthy volunteers as
previously described(G. Li, Ma, et al.,
2018). Approximately 200 μL of plasma was prepared in an ice-cold
phosphate-buffered solution. Protein levels of TNF-α, IL-1β, and MMP-9
were quantified using a commercial ELISA kit (Abcam), following the
manufacturer’s instructions.