Background: Hematuria is one of the most common findings in urology. The management of hematuria detected in routine medical check-ups is also important in this respect. The aim of this study was to determine the frequency of hematuria in patients undergoing medical check-ups and evaluate its association with abnormal urological findings in laboratory tests and imaging. Methods: Asymptomatic patients who presented to the check-up clinic between January 1 and December 31, 2020 were included. All patients underwent a complete laboratory workup and total abdominal ultrasound (TAUS). The distributions of hematuria by sex and age and the relationship between hematuria and abnormal urological findings in the laboratory tests and TAUS were calculated. Results: The mean age of the 2077 check-up patients was 45.8±12.8 years. The female to male ratio was 1.07:1 and there was no significant difference in age between the sexes (p=0.655). Microscopic hematuria was detected in 15.9% of the patients. The frequency of hematuria was 10.8% in men and 20.7% in women (p<0.001). Men with hematuria were significantly older than men without hematuria (p=0.033), while hematuria was not associated with age in women (p=0.521). When its relationship between abnormal urological findings was evaluated, hematuria had sensitivity of 21.70% (95% CI: 18.84, 24.86) and specificity of 87.11% (95% CI: 85.23, 88.79). Conclusion: The frequency of hematuria in check-up patients was remarkable, at 15.9%. Hematuria in women is more common and unrelated to age, unlike in men. Hematuria had high specificity but low sensitivity for urological abnormal findings.