Figure 2. Experimental setup and image analysis.
We sterilized the DEP chip using 70% isopropyl alcohol and then rinsed with deionized water. Next, we introduced the DEP buffer to remove bubbles from the electrode array. Then, we loaded 40-µl cell suspension into the chip using a 200 µl pipette tip. The cells were settled in the electrode array for 30 seconds and there was no flow during the experiments. The signal with 3 Vpp and 10 kHz - 10 MHz was applied using the function generator.