3.2 Dielectrophoretic Behavior of U87 Glioma Cells
We characterized the dielectrophoretic properties of U87 glioma cells by applying 3 Vpp with varying frequencies between 100 kHz – 10 MHz nonuniform electric field for 8 minutes, as shown inFigure 2 . We introduced 40-µl cell solution into the device. When the flow of the cells due to potential difference between the inlet and outlet levelled, we switched the electric field on using 3 Vpp, 50 kHz. We waited for 30 seconds for each frequency values and then increased the frequency as 100 kHz, 200 kHz, 400 kHz, 500 kHz, 1 MHz, 2 MHz, 5 MHz, 10 MHz. We captured the images of the cells with 5-frame/second frame rate. We manually analyzed images using ImageJ software as explained above. We determined the location of the cells in the electrode array for each frequency and recorded their position whether the cell exhibits nDEP or pDEP, as illustrated inFigure 4, Figure 5 .