Hematoxylin-eosin staining of placental arterioles
After fixation, the placental arterioles were dehydrated by an ethanol
gradient and cleared with xylene. The transparent tissue blocks were
embedded in melted paraffin, and the tissue blocks were then sliced and
baked after being cooled and solidified. The dried slices were dewaxed
by xylene, dehydrated by gradient ethanol, stained by hematoxylin and
eosin and finally dehydrated, made transparent, sealed and imaged.