Human umbilical vein endothelial cell culture
After resuscitation, HUVECs (Fuxiang Biotechnology Co., LTD, Shanghai,
CN) were cultured in high-glucose DMEM containing 10% fetal bovine
serum and 1% penicillin and streptomycin at 5% CO2 and
37 °C in a humidified chamber. HUVECs at the logarithmic growth stage
were subcultured and inoculated in six-well plates. The cells were
divided into the control group, capsaicin group and capsazepine (MCE,
USA) group. After approximately 80% of the cells were fused,
high-glucose DMEM containing capsaicin and capsazepine, which were
prepared in DMSO at a final concentration of 1 µmol/L, was administered,
and ordinary high-sugar DMEM containing an equal volume of DMSO was
administered to the control group. After 24 hours of incubation, total
RNA and protein were extracted from the cells.