Human umbilical vein endothelial cell culture
After resuscitation, HUVECs (Fuxiang Biotechnology Co., LTD, Shanghai, CN) were cultured in high-glucose DMEM containing 10% fetal bovine serum and 1% penicillin and streptomycin at 5% CO2 and 37 °C in a humidified chamber. HUVECs at the logarithmic growth stage were subcultured and inoculated in six-well plates. The cells were divided into the control group, capsaicin group and capsazepine (MCE, USA) group. After approximately 80% of the cells were fused, high-glucose DMEM containing capsaicin and capsazepine, which were prepared in DMSO at a final concentration of 1 µmol/L, was administered, and ordinary high-sugar DMEM containing an equal volume of DMSO was administered to the control group. After 24 hours of incubation, total RNA and protein were extracted from the cells.