Coloration analyses
The quantification of plumage chromatic distances between females ofT. r. ruficapillus (from the Atlantic Forest) and T. r.
cochabambae (from the Andean forest) using the Vorobyev and Osorio
(1998) model indicated that five plumage patches had differences in
colour above the discrimination threshold (∆S > 1.00 jnd):
throat, breast, belly, crown and nape (Table 2). In turn, the comparison
between males of these same two subspecies showed differences higher
than 1.00 jnd for three plumage patches: throat, belly and nape (Table
2). The PERMANOVA, however, was not significant for any of the
comparisons (Table 2), suggesting that the variation between subspecies
was not significantly higher than the variation within each of them
neither for females nor for males. This relatively subtle,
non-significant colour differentiation between subspecies can also be
observed in the reflectance spectra of the patches (Figure S5).