DIl-ac-LDL assay
In 24-well plates, T-ECs and N-ECs were cultivated and then incubated
for 48 hours. Following this, a serum-free ECM medium was used to
replace the previous medium, which was allowed to incubate for an
additional 3 hours. Afterward, Dil-ac-LDL, which had been labeled with
rhodamine and prepared from serum-free ECM medium, was introduced into
the wells. Once a 4-hour incubation period had passed, the medium was
again aspirated. To remove any unbound Dil-ac-LDL, the wells were then
rinsed with 0.01 M PBS. Using a fluorescence microscope, the results of
the experiment were then examined.