In vivo tumor biology
Eight NOD/SCID mice, each four weeks old and weighing around 20±2 grams,
were procured from Beijing Vital River Laboratory Animal Technology Co.,
Ltd. These animals were placed in a facility with a controlled
environment of 22±1˚C temperature, 55±2% humidity, and a 12/12‑h
light/dark cycle, with unlimited water and food access. Each mouse
received a subcutaneous injection of 0.2 ml PBS containing 5×106 HepG2
cells on its right side. Post a fortnight, the mice were given an
intravenous injection of either miR‑205‑3p antagomir (100 nmol) or 200
µl antagomir NC in the tail. Every five days, a slide caliper was
utilized to measure the size of the tumor. Following 42 days from the
initial injection, Intraperitoneal administration of a mix of 100 mg/kg
ketamine and 10 mg/kg xylazine anesthetized the mice. After euthanizing
the mice via cervical dislocation, the volume of their tumors was
measured. The presence of Ki67 was determined using immunofluorescence.