Figure 4. Identifying HINT1 as a miR-205-3p target gene.
MiR-205 3p’s putative target location in the 3’UTR of HINT1. (B) The relative luciferase activity was measured 48 hours after co-transfection of HepG2 and HepB3 cells with a miR NC or miR-205 3p inhibitor and a luciferase reporter vector expressing either the WT or the MUT 3’UTR of HINT1. *P<0.05 vs. miR NC. (C) The evaluation of mRNA expression levels was conducted. The data represents the mean ± SD from three separate tests. **P<0.01. Terminology: MUT, mutant; NC, negative control; WT, wild type; miR, microRNA.