CD105+ cell purification and
identification of endothelial function
An adapted technique for the purification of vascular endothelial cells
was utilized to gather vascular endothelial cells
(CD105+ cells), independently from both liver cancer
tissue and normal liver tissue. The liver cancer vascular endothelial
cells (T-ECs, Tumor-Endothelial Cells) and normal hepatic vascular
endothelial cells (N-ECs, Normal-Endothelial Cells) microscopically
showed a goose-flint-like morphology (Fig. 1A). Based on flow cytometry,
CD105 positive rates of T-ECs and N-ECs were 95.6 ± 1.4 % and 94.9 ±
2.3 %, respectively (Fig. 1B), increasing the sorting efficiency of
positive cells to more than 95 % to minimize the effect of negative
cells on subsequent experiments, using endothelial cell tube-forming
assay and endothelial cell. The endothelial cell function of T-ECs and
N-ECs was identified using endothelial cell tube formation and
endothelial cell phagocytosis assays. As a result, we discovered that
T-ECs and N-ECs could form tubular lumen-like structures on Matrigel gel
(Fig. 1C) and phagocytosed ac-LDL before emitting red fluorescence (Fig.
1D).