In vivo tumor biology
Eight NOD/SCID mice, each four weeks old and weighing around 20±2 grams, were procured from Beijing Vital River Laboratory Animal Technology Co., Ltd. These animals were placed in a facility with a controlled environment of 22±1˚C temperature, 55±2% humidity, and a 12/12‑h light/dark cycle, with unlimited water and food access. Each mouse received a subcutaneous injection of 0.2 ml PBS containing 5×106 HepG2 cells on its right side. Post a fortnight, the mice were given an intravenous injection of either miR‑205‑3p antagomir (100 nmol) or 200 µl antagomir NC in the tail. Every five days, a slide caliper was utilized to measure the size of the tumor. Following 42 days from the initial injection, Intraperitoneal administration of a mix of 100 mg/kg ketamine and 10 mg/kg xylazine anesthetized the mice. After euthanizing the mice via cervical dislocation, the volume of their tumors was measured. The presence of Ki67 was determined using immunofluorescence.