MTT assay
The MTT test was employed to ascertain cell viability.
Post-transfection, 96-well plates were used to culture the cells with
each well containing 1.0x104 cells and subsequently
incubated at 37˚C for time intervals of 24h, 48h, and 72h. Next, a 0.5
mg/ml MTT solution (Sigma; Merch KGaA) was introduced into the cells and
left to culture for 4 hrs, immediately after which co-culturing with
100µl DMSO was performed at 37˚C for 10 min. The optical density was
subsequently calculated from the absorbance measurements taken at 490
nm.