Figure 4 Macrophages were decreased by treat with EGCG in colon of the UC mice. Immunohistochemisty was performed on paraffin sections stained for F4/80 and
iNOS(A). The Brown area (red arrows) indicates positive staining. The tissue was obtained from the colon of mice was homogenized in protein extraction solution and 60 μg for each well for detected. The protein expressions of iNOS (B) were measured by western blotting. The value of OD (C) was detected by Image J (NIH). Data are pooled from two experiments (n=6 colon from six mice per group, repeated twice). Data are presented as the means ± SEM. *P<0.05, **P<0.01, ***P<0.001 (one-way ANOVA, EGCG, and normal group vs. DSS group). Scale bars, 100 μm. LE, Low dose of (-)-epigallocatechin gallate; HE, High dose of (-)-epigallocatechin gallate; DSS, Dextran sulfate sodium.