EGCG reduced the level of inflammatory cytokines in colon
tissues of DSS-treated mice
The increased inflammation cytokines, such as TNF-α, IL-1β, IL-6, MCP-1,
plays a critical role in DSS-induced ulcerative
colitis4. To investigate the effect of EGCG on
inflammation-associated molecules, ELISA assay was performed to confirm
the effects of EGCG on the protein levels of TNF-α, IL-1β, IL-6 and
MCP-1. Compared with the Normal group, these cytokines in colonic tissue
were upregulated markedly in colonic tissues of mice with oral
administration of DSS (figure3A, 3B, 3C and 3D). While secretion levels
of these inflammatory cytokines were significantly suppressed in mice
treated with EGCG (figure3A, 3B, 3C and 3D). Furthermore, we evaluated
the expression levels of TNF-α and IL-1β using western blot assays. The
levels of TNF-α and IL-1β was significantly reduced by EGCG treat
compare with those of the DSS treatment group (figure 3E, 3F and 3G).
Taken together, these data indicated that EGCG could inhibit the
overwhelming inflammatory response in colonic tissue of mice with
DSS-induced colitis.