Figure 3 The level of cytokine secretion and protein expression can be inhibited by EGCG in colon of the UC mice. Colon in UC mice was treated with EGCG or placebo and harvested at day 8 post DSS induced. To measure the pro-inflammatory marker TNF-α (A), IL-1β (B), MCP-1 (C), and IL-6 (D), tissues were treated as described earlier using ELISA. The tissue was obtained from the colon of mice was homogenized in protein extraction solution and 60 μg for each well for detected. The protein expressions of TNF-α and IL-1β (E) were measured by western blotting. The value of OD (F, G) was detected by Image J (NIH). For all graphs, bars = mean ± SEM, n = 5. Statistical analysis was performed using one-way ANOVA. ***Mean value was significantly different for the EGCG and normal group at the same time point compared with the model group. LE, Low dose of (-)-epigallocatechin gallate; HE, High dose of (-)-epigallocatechin gallate; DSS, Dextran sulfate sodium.