Figure 1 EGCG could
attenuate inflammatory responses by targeting the Notch pathway in
Caco-2 cells. The supernatant and samples were obtained from the Caco-2
cell line after LPS stimulation with or without EGCG treatment for 24 h.
To measure the pro-inflammatory marker TNF-α, the supernatant was
treated as described earlier using ELISA. 60 μg of each sample was
applied to each well for detection of inflammation protein and Notch
signaling pathway protein. (A ) TNF-α was detected to
demonstrate whether EGCG attenuates inflammatory response in Caco-2
cells. (B, C ) The protein expression of COX2 was measured by
western blotting, and the ODs were determined. (D ) The protein
levels of Notch1, Notch2, Cleaved-Notch1, and Hes-1 were detected by
western blotting, and the ODs were calculated to demonstrate the effect
of EGCG on the Notch signaling pathway in the Caco-2 cell. Data are
pooled from two experiments. Data are presented as the means ± SEM.
*P<0.05, **P<0.01, ***P<0.001 (one-way
ANOVA). EGCG, (-)-epigallocatechin gallate; LPS, Lipopolysaccharide.