Macrophage phenotype could change by administration of EGCG in
colitis mice
In our previous study, the macrophage phenotype could be changed with
EGCG treat17. To further understand the potential
mechanism by which EGCG therapy mitigate intestinal inflammation in this
study, we analyzed the macrophages accumulation and the polarization of
M1 macrophage by IHC and western blot analyses. The results showed that
the levels of F4/80, which is marker of macrophages, were increased by
DSS treat compare with normal group. And EGCG treatment could attenuate
the macrophages accumulated (figure 4A). More importantly, the nitric
oxide synthase (iNOS, M1 specific mark) were down-regulated with EGCG
treat (figure 4A). The protein of iNOS expression was also decreased by
EGCG treatment compared with mice in the DSS group (figure 4B and 4C).
These results show that EGCG resolve inflammation of the colons partly
because of reduced macrophages, especially the type of M1 macrophages.