Figure legends
Figure 1. Volcano plot of DEGs between B-ALL patients and normal samples. Statistically significant DEGs were defined with adjusted P -value < 0.05 and absolute log2 FC > 2.0 as the cut-off threshold.
Figure 2. Weighted Gene Co-Expression Network Analysis (WGCNA) to identify Gene Modules. (A) The clustering dendrogram of differentially expressed genes in B-ALL. A total of 18 co-expression modules were identified, each leaf (vertical line) in the dendrogram was assigned to one co-expression gene, and the color row below the dendrogram represents the modules. The SPRING1 was clustered in the turquoise module, (B) Heatmap plot of the adjacencies of modules. Red represents a positive correlation, and green represents a negative correlation. The amount of correlation and the calculatedP -values are also shown in each cell. The module assignment is shown on the left. The yellow, blue, turquoise, and magenta modules have the highest correlation with B-ALL cancer. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Figure 3. (A) Box plot representing a descriptive analysis of relative gene expression of SPRING1 in B-ALL patients and ITP samples; (B) Mean of log2 fold change for the SPRING1 mRNA expression.
Figure 4. ROC curve of SPRING1 for diagnosis of B-ALL patients. The AUC value differs from 0 to 1, which indicates the diagnostic power based on favorable (0.9-1.0), appropriate (0.8-0.9), acceptable (0.7-0.8), weak (0.6-0.7), and less (0.5-0.6). ROC: receiver operating characteristic; AUC: area under the ROC curve.
Figure 5. Validating the role of SPRING1 in lymphoid disorder by the GEPIA dataset. (A) Box plot shows expression ofSPRING1 in cancer (left) and normal (right) samples; (B) Stage plot shows SPRING1 expression in 4 stages; (C) Overall survival between patients with the higher (red) and lower (blue) SPRING1expression.