Figure legends
Figure 1. Volcano plot of DEGs between B-ALL patients and
normal samples. Statistically significant DEGs were defined with
adjusted P -value < 0.05 and absolute log2 FC
> 2.0 as the cut-off threshold.
Figure 2. Weighted Gene Co-Expression Network Analysis (WGCNA)
to identify Gene Modules. (A) The clustering dendrogram of
differentially expressed genes in B-ALL. A total of 18 co-expression
modules were identified, each leaf (vertical line) in the dendrogram was
assigned to one co-expression gene, and the color row below the
dendrogram represents the modules. The SPRING1 was clustered in
the turquoise module, (B) Heatmap plot of the adjacencies of
modules. Red represents a positive correlation, and green represents a
negative correlation. The amount of correlation and the calculatedP -values are also shown in each cell. The module assignment is
shown on the left. The yellow, blue, turquoise, and magenta modules have
the highest correlation with B-ALL cancer. (For interpretation of the
references to color in this figure legend, the reader is referred to the
web version of this article.)
Figure 3. (A) Box plot representing a descriptive analysis of
relative gene expression of SPRING1 in B-ALL patients and ITP
samples; (B) Mean of log2 fold change for the SPRING1 mRNA
expression.
Figure 4. ROC curve of SPRING1 for diagnosis of B-ALL
patients. The AUC value differs from 0 to 1, which indicates the
diagnostic power based on favorable (0.9-1.0), appropriate (0.8-0.9),
acceptable (0.7-0.8), weak (0.6-0.7), and less (0.5-0.6). ROC: receiver
operating characteristic; AUC: area under the ROC curve.
Figure 5. Validating the role of SPRING1 in lymphoid
disorder by the GEPIA dataset. (A) Box plot shows expression ofSPRING1 in cancer (left) and normal (right) samples; (B) Stage
plot shows SPRING1 expression in 4 stages; (C) Overall survival between
patients with the higher (red) and lower (blue) SPRING1expression.