4.10. Fluorescence Imaging of Mice Tissue
The control group sham surgery group. Orthotopic mouse models of HCC
were randomly divided into several groups (n=5). Mice was injected with
50 μL 10 μM TPEARG PBS solution (1% DMF) and 50 μL 20 μM Cy-FAP PBS
solution for 30 minutes via tail veins, respectively. Then liver tissues
of each mouse were sliced using Leica CM1950. Fluorescence imaging of
mice tissues were carried out using laser scanning microscope with a
blue channel (λex=405 nm (intensity=8%),
λem=410 nm- 480 nm) for TPEARG and a red channel
(λex=633 nm (intensity=10%), λem=700
nm- 780 nm) for Cy-FAP.