4.9. Fluorescence Imaging of Cells In Vitro
RAW 264.7 and LX-2 cells were seeded on the round coverslips in 6-well plates and then treated cells with different reagents. For arginase imaging, cells incubated with 100 μL 1 μM TPEARG PBS solution (1% DMF) for 30 minutes. Then the cells were washed with PBS 3 times, and imaged under the confocal laser scanning microscope with a blue channel (λex=405 nm (intensity=5%), λem=410 nm- 480 nm). For FAP imaging, cells were incubated with 100 μL 20 μM Cy-FAP PBS solution for 30 minutes. Then the cells were washed with PBS 3 times, and imaged under the confocal laser scanning microscope with a red channel (λex=633 nm (intensity=6%), λem=700 nm- 780 nm) for Cy-FAP.