4.5. Cell Culture
Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10%
heat-inactivated fetal bovine serum and 1% penicillin and streptomycin
was used to culture Raw 264.7 and LX-2 cells. Cells were grown at 37 °C
in a humidified environment of 5% CO2. Medium
replacements were made one or two days. LX-2 cells were collected using
pancreatin and Raw 264.7 cells were collected by DMEM washing.