4.7. In Vivo Toxicity Assays
C57BL/6J mice (male, 4-6 weeks) were housed in a pathogen-free, temperature controlled facility with 12-hour light/dark cycles. Animals received food and water ad libitum.
Mice were divided into two groups (6 mice per group). The mice in control group were given 200 μL PBS (pH=7.4) solution via intravenous injection per day for 7 days. The mice in experimental group were given 200 μL 200 μM TPEARG PBS solution (pH=7.4) via intravenous injection per day for 7 days. Their body weights were recorded every day. At the end of the experiment, major organs of heart, liver, spleen, lung and kidney in mice of each group were obtained and stained with hematoxylin and eosin (H&E) to assess toxicity.