4.6. Cytotoxicity Assays
Raw 264.7 cells were seeded in two 96-well plates at a concentration of 1×105 and cultured for 12 h. In one 96-well plates, cells were cultured with 0, 500 nM, 50 μM, 100 μM, 200 μM, 500 μM TPEARG for 12 h. The medium volume of every well was 100 μL. Then, 10 μL CCK-8 solution was added to each well and incubated for another 1 h at 37 °C. After that, the absorbance at 450 nm was detected in a Triturus microplate reader.