4.5. Cell Culture
Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin and streptomycin was used to culture Raw 264.7 and LX-2 cells. Cells were grown at 37 °C in a humidified environment of 5% CO2. Medium replacements were made one or two days. LX-2 cells were collected using pancreatin and Raw 264.7 cells were collected by DMEM washing.