Figure 5. Activation of HSCs by arginase in mice. (A) Cy-FAP and TPEARG were applied in the normal mice (Control) and orthotopic mouse models of HCC treated with LPS (Arginase agonist) and BEC (Arginase inhibitor). (B) Western blot of CTLA4 in each mouse group. ELISA Kit test of the expressions of immune evasion markers PD-L1 (C), TNF-α(D), IL-10 (E) and IFN-γ (F). The data are expressed as mean ± SD. n = 3. Scale bar= 25 µm.