4.2. Instruments
UV-2600 UV-visible spectrophotometer (Shimadzu, Japan) was utilized to analyze absorption spectra. The F-4600 HITACHI fluorescence spectrophotometer was used to detect the fluorescence spectra. Utilizing the Bruker maXis ultra-high resolution-TOF MS equipment, mass spectra were identified. Using Bruker NMR spectrometers, 1H NMR spectra were measured at 400 MHz, while 3C NMR spectra were recorded at 100 MHz. Using a Leica TCS SP8 confocal laser scanning microscope, fluorescence imaging of cells was performed. Using the Perkinelmer IVIS Lumina III, in vivo NIR imaging of mice was measured.