4.2. Instruments
UV-2600 UV-visible spectrophotometer (Shimadzu, Japan) was utilized to
analyze absorption spectra. The F-4600 HITACHI fluorescence
spectrophotometer was used to detect the fluorescence spectra. Utilizing
the Bruker maXis ultra-high resolution-TOF MS equipment, mass spectra
were identified. Using Bruker NMR spectrometers, 1H NMR spectra were
measured at 400 MHz, while 3C NMR spectra were recorded at 100 MHz.
Using a Leica TCS SP8 confocal laser scanning microscope, fluorescence
imaging of cells was performed. Using the Perkinelmer IVIS Lumina III,
in vivo NIR imaging of mice was measured.