Material and methods
Taxonomy: The morphological analysis and description of the newly discovered species were made through examination of live specimens on the field as well as in the laboratory. Herbarium specimens housed at BLAT, BSI, CAL and MH were studied. Also, the specimens were compared with the type specimens of closely related species in the virtual herbaria (K and E). The acronyms of the herbaria consulted is as per Index Herbariorum (http://sweetgum.nybg.org/science/ih/). Descriptions were made following the terminology of Hickey and King (2001).
Cytogenetics: Mitotic studies of the new species and S .bombayensis were conducted on the root tips obtained from germinating cypselae. Root tips were pre-treated with a saturated solution of para -Dicholorobenzene (p DB) for 4–5 h at 7±2℃. The roots tips were then hydrolysed in 1N HCl and squashed in 2% propionic orcein. Suitable plates from freshly prepared slides were photographed with Leica DM 750 microscope with attached camera at 1000X magnification. Five cells with well-spread metaphase chromosomes were selected for karyotype analysis by using KaryoMeasure software (Mahmoudi and Mirzaghaderi 2021). Karyological parameters, viz. 2n , mean chromosome length (MCL), total haploid chromosome length (THL), mean centromeric asymmetry (MCA), coefficient of variation of chromosome length (CVCL) and coefficient of variation of centromeric index (CVCI) were used for karyotype analysis. To generate chromosomes with secondary constriction in Idiogram, single image was selected for measurement in Idiogram generation option found in the side panel of software. Voucher specimens were deposited in the Herbarium of Shivaji University, Kolhapur (SUK).