Material and methods
Taxonomy: The morphological analysis and description of the newly
discovered species were made through examination of live specimens on
the field as well as in the laboratory. Herbarium specimens housed at
BLAT, BSI, CAL and MH were studied. Also, the specimens were compared
with the type specimens of closely related species in the virtual
herbaria (K and E). The acronyms of the herbaria consulted is as per
Index Herbariorum (http://sweetgum.nybg.org/science/ih/). Descriptions
were made following the terminology of Hickey and King (2001).
Cytogenetics: Mitotic studies of the new species and S .bombayensis were conducted on the root tips obtained from
germinating cypselae. Root tips were pre-treated with a saturated
solution of para -Dicholorobenzene (p DB) for 4–5 h at
7±2℃. The roots tips were then hydrolysed in 1N HCl and squashed in 2%
propionic orcein. Suitable plates from freshly prepared slides were
photographed with Leica DM 750 microscope with attached camera at 1000X
magnification. Five cells with well-spread metaphase chromosomes were
selected for karyotype analysis by using KaryoMeasure software (Mahmoudi
and Mirzaghaderi 2021). Karyological parameters, viz. 2n , mean
chromosome length (MCL), total haploid chromosome length (THL), mean
centromeric asymmetry (MCA), coefficient of variation of
chromosome length (CVCL) and coefficient of variation of
centromeric index (CVCI) were used for karyotype
analysis. To generate chromosomes with secondary constriction in
Idiogram, single image was selected for measurement in Idiogram
generation option found in the side panel of software. Voucher specimens
were deposited in the Herbarium of Shivaji University, Kolhapur (SUK).