Material and Method
1.1 Knockout of the SDRC protein sequence of Staphylococcus aureus
The standard strain ATCC25923 of Staphylococcus aureus used in this
study was purchased from the bacterial library of the Chinese Academy of
Sciences. Genetic engineering technology was used to knock out the Sdrc
protein region in Staphylococcus aureus. First, design upstream and
downstream homologous arm sequence primers for SDRC protein based on the
genome sequence of Staphylococcus aureus (Primer Schedule 1). And
through overlapping PCR amplification, mutant plasmid construction,
plasmid transformation, cloning, and other operations, we ultimately
successfully constructed a mutant strain of Staphylococcus aureus with
SDRC knockout. And we further validated the knockout effect of strain
SDRC using next-generation sequencing technology.
Schedule 1 The primers of SDRC knockout primers