Material and Method
1.1 Knockout of the SDRC protein sequence of Staphylococcus aureus
The standard strain ATCC25923 of Staphylococcus aureus used in this study was purchased from the bacterial library of the Chinese Academy of Sciences. Genetic engineering technology was used to knock out the Sdrc protein region in Staphylococcus aureus. First, design upstream and downstream homologous arm sequence primers for SDRC protein based on the genome sequence of Staphylococcus aureus (Primer Schedule 1). And through overlapping PCR amplification, mutant plasmid construction, plasmid transformation, cloning, and other operations, we ultimately successfully constructed a mutant strain of Staphylococcus aureus with SDRC knockout. And we further validated the knockout effect of strain SDRC using next-generation sequencing technology.
Schedule 1 The primers of SDRC knockout primers