Figure Attachment 2: Construction of a stable osteoclast transfection strain interfered by Myh7. A: WB screening for the best interfering vector to β- Actin is an internal reference protein. B: RT-PCR validation of the optimal interference sequence, using GAPDH as the internal reference. C: The lentivirus was transfected into 293T cells for packaging (100X). D: Chronic virus infection of osteoclasts (100X).