PCR Amplification and Sequencing
The PCR was performed in a 20-µl reaction volume [1 µl of DNA template (5 ng/µl), 1 µl each of the forward and reverse primer (10 pmol/µl), 7 µl of ddH2O, and 10 µl of 2×Mei5bio Taq PCR mix]. The amplification conditions were as follows: 5 min at 95℃, followed by 35 cycles of 30 s at 95℃, 30 s at 58℃ (56℃), 30 s at 72℃, with a final extension of 5 min at 72℃, stored at 4℃. The PCR products were run on a 1.5% agarose gel. Double-ended sequencing was executed on ABI 3730 automated sequencer.