cDC activation
For in vitro activation assays, cDCs were isolated and incubated
overnight in complete RPMI with or without 0.5 µM CpG 1668 ODN (Bioneer)
at 37ºC or 4ºC. For in vivo activation assays, mice were injected
intravenously with 0.2 µM CpG, 3 µg LPS, 50 µg
polyinosinic:polycytidylic acid (poly I:C) or PBS.