In this study, we introduce datasets that include both hydrological and meteorological records at the Nučice experimental catchment (0.53 km2) which is representative for an intensively farmed landscape in the Czech Republic. The Nučice experimental catchment was established in 2011 for the observation of rainfall-runoff processes, soil erosion processes, and water balance of a cultivated landscape. The average altitude is 401 m a.s.l., the mean land slope is 3.9%, and the climate is humid continental (mean annual temperature 7.9 °C, annual precipitation 630 mm). The catchment is drained by an artificially straightened stream and consists of three fields covering over 95 % of the area which are managed by two different farmers. The typical crops are winter wheat, rapeseed, and alfalfa. The installed equipment includes a standard meteorological station, several rain gauges distributed across the basin, and an H-flume that monitors stream discharge, water turbidity, and basic water quality indicators. Additionally, the groundwater level and soil water content at various depths near the stream are recorded. Recently, large-scale soil moisture monitoring efforts have been introduced with the installation of two cosmic-ray soil moisture sensors. The datasets consist of measured precipitation, air temperature, stream discharge, and soil moisture and are available online for public use. The cross seasonal, open access runoff generation datasets at this small-scale agricultural catchment will benefit not only hydrologists but also local farmers.